Studies Referencing Hydrogen and Mouth/Teeth

Database:

Primary Topic: Mouth

Secondary Topic: Cavities/Dentin Integrity/Denture Care/Periodontitis/Gingivitis (or Any)

Tertiary Topic: Root Canal (or Any)

Oral and Tooth Care

● Hydrogen has been shown to improve oral and tooth health by

• improving Candida yeast infection (226: Park et al 2019; 557: Pyo et al 2015),
• cavities (397: Kim et al 2017),
• denture care (234: Song and Lee 2019; 847: Kim, Choi, and Cho 2011),
• gingivitis (443: Xiao and Miwa 2017; 710: Lee and Baek 2013; 454: Yoneda et al 2017),
• root canal efficacy (1008: Horiba et al 1999; 172: Chen et al 2019),
• periodontitis (328: Rahardjo, Ridwan, and Hikmah 2018; 363: Zhou and Wang 2018; 425: Ridwan, Juliastuti, and Setijanto 2017; 845: Kasuyama et al 2011; 668: Tomofuji et al 2014; 132: Vahabi, Shokri, and Lazar 2020; 537: Azuma et al 2015; 864: Okajima et al 2011; 974: Lee and Choi 2006; 1033: Nayak et al 2021; 1102: Saitoh et al 2021),
• and oral wound healing (519: Tamaki et al 2016).

Cancer

● Hydrogen has shown efficacy as a carcinostatic in

• oral (29: Takemoto et al 2021; 306: Kusinoki et al 2018),
• tongue (81: Kato, Saitoh, and Miwa 2020; 945: Saitoh et al 2009; 959: Saitoh et al 2008),
• and esophageal (12: Kato, Saitoh, and Miwa 2021; 81: Kato, Saitoh, and Miwa 2020; 403: Li, Tanaka, and Miwa 2017) cancers.

Mode of Action

Hydrogen shows multiple modes of action, including quenching cellular ROS (443: Xiao and Miwa 2017; 454: Yoneda et al 2017; 363: Zhou and Wang 2018; 425: Ridwan, Juliastuti, and Setijanto 2017; 845: Kasuyama et al 2011; 668: Tomofuji et al 2014),

increasing the activity of endogenous antioxidant enzymes (363: Zhou and Wang 2018; 537: Azuma et al 2015),

upregulating cellular protection mechanisms (519: Tamaki et al 2016),

counteracting inflammation and apoptosis (845: Kasuyama et al 2011; 1102: Saitoh et al 2021),

and improving immune function (328: Rahardjo, Ridwan, and Hikmah 2018).

Literature Cited (numbered as per database):

● (12) Kato, Shinya; Saitoh, Yasukazu; Miwa, Nobuhiko. Carcinostatic effects of alkanoyl ascorbate plus platinum nano-colloid and stabilization of the esterolytically resultant ascorbate by hydrogen. 2021. Human Cell. 10.1007/s13577-020-00462-3

● (29) Takemoto, Tomoko; Kaetsu, Ryo; Hanayama, Machiko; Ishiyama, Yuuichi; Sadamura, Masayuki; Nishio, Kensuke; Tsunoda, Mariko; Asano, Masatake; Motoyoshi, Mitsuru. Acid-electrolyzed functional water-induces Interleukin-1α release from Intracellular Storage Sites in Oral Squamous Cell Carcinoma. 2021. International Journal of Medical Sciences. 10.7150/ijms.53999

● (81) Kato, Shinya; Saitoh, Yasukazu; Miwa, Nobuhiko. Hydrogen-bubbled platinum-colloid suppresses human esophagus- or tongue-carcinoma cells with intracellular platinum-uptake and the diminished normal-cell mortality. 2020. Human Cell. 10.1007/s13577-020-00402-1

● (132) Vahabi, Surena; Shokri, Mehdi; Lazar, Masih. Effects of Electrolyzed Water on the Growth of Oral Pathologic Bacteria Species and its Cytotoxic Effects on Fibroblast and Epithelial Cells at Different pH Values. 2020. Iranian Journal of Medical Sciences. 10.30476/ijms.2019.45392

● (172) Chen, Ker-Kong; Wu, Ju-Hui; Wei, Shin-I.; Du, Je-Kang. Influence of the acidity of electrolyzed water on the microhardness of inner layer dentin. 2019. Journal of Dental Sciences. 10.1016/j.jds.2019.09.007

● (226) Park, Mi-Jung; Choi, Youn-Hee; Song, Keun-Bae; Kim, Eun-Kyong. Antifungal effects of hydrogen water on the growth of Candida albicans. 2019. Journal of Korean Academy of Oral Health. 10.11149/jkaoh.2019.43.4.173

● (234) Song, Young-Gyun; Lee, Sung-Hoon. Efficacy of newly developed denture cleaning device on physical properties of denture material and Candida biofilm. 2019. Journal of Dental Sciences. 10.1016/j.jds.2019.01.011

● (306) Kusinoki, Masafumi; Sata, Eri; Nishio, Kensuke; Tanaka, Takayoshi; Nishida, Tetsuya; Sugano, Naoyuki; Sato, Shuichi; Asano, Masatake. Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines. 2018. International Journal of Medical Sciences. 10.7150/ijms.26186

● (328) Rahardjo, Markus B.; Ridwan, Rini D.; Hikmah, Delaneira A.D. Effect of electrolyzed reduced water on malondialdehyde levels and neutrophil cells in aggressive periodontitis. 2018. Tropical Journal of Pharmaceutical Research. 10.4314/tjpr.v17i2.6

● (363) Zhou, Min; Wang, Zuo-Lin. Protective effects of hydrogen-rich medium on lipopolysaccharides-induced injury in human periodontal ligament cells. 2018. West China Journal of Stomatology. 10.7518/hxkq.2018.02.002

● (397) Kim, Jinkyung; Lee, Heon-Jin; Hong, Su-Hyung. Inhibition of streptococcal biofilm by hydrogen water. 2017. Journal of Dentistry. 10.1016/j.jdent.2017.01.004

● (403) Li, Qiang; Tanaka, Yoshiharu; Miwa, Nobuhiko. Influence of hydrogen-occluding-silica on migration and apoptosis in human esophageal cells in vitro. 2017. Medical Gas Research. 10.4103/2045-9912.208510

● (425) Ridwan, Rini Devijanti; Juliastuti, Wisnu Setyari; Setijanto, R. Darmawan. Effect of electrolyzed reduced water on Wistar rats with chronic periodontitis on malondialdehyde levels. 2017. Dental Journal. 10.20473/j.djmkg.v50.i1.p10-13

● (443) Xiao, Li; Miwa, Nobuhiko. Hydrogen-rich water achieves cytoprotection from oxidative stress injury in human gingival fibroblasts in culture or 3D-tissue equivalents, and wound-healing promotion, together with ROS-scavenging and relief from glutathione diminishment. 2017. Human Cell. 10.1007/s13577-016-0150-x

● (454) Yoneda, Toshiki; Tomofuji, Takaaki; Kunimoto, Muneyoshi; Ekuni, Daisuke; Irie, Koichiro; Azuma, Tatsuji; Machida, Tatsuya; Miyai, Hisataka; Fujimori, Kouhei; Morita, Manabu. Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet. 2017. Nurtients. 10.3390/nu9010064

● (519) Tamaki, Naofumi; Orihuela-Campos, Rita Cristina; Fukui, Makoto; Ito, Hiro-O. Hydrogen-Rich Water Intake Accelerates Oral Palatal Wound Healing via Activation of the Nrf2/Antioxidant Defense Pathways in a Rat Model. 2016. Oxidative Medicine and Cellular Longevity. 10.1155/2016/5679040

● (537) Azuma, Tetsuji; Yamane, Mayu; Ekuni, Daisuke; Kawabata, Yuya; Kataoka, Kota; Kasuyama, Kenta; Maruyama, Takayuki; Tomofuji, Takaaki; Morita, Manabu. Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study. 2015. Antioxidants. 10.3390/antiox4030513

● (577) Pyo, Kyung-Ryul; Yoo, Yun Seung; Baek, Dong-Heon. Antifungal effect of electrolyzed hydrogen water on Candida albicans biofilm. 2015. Journal of Dental Rehabilitation and Applied Science. 10.14368/jdras.2015.31.3.212

● (668) Tomofuji, Takaaki; Kawabata, Yuya; Kasuyama, Kenta; Endo, Yasymasa; Yoneda, Toshiki; Yamane, Mayu; Azuma, Tatsuji; Ekuni, Daisuke; Morita, Manabu. Effects of hydrogen-rich water on aging periodontal tissues in rats. 2014. Nature Scientific Reports. 10.1038/srep05534

● (710) Lee, Sung-Hoon; Baek, Dong-Heon. Antibacterial Activity of Hydrogen-rich Water Against Oral Bacteria. 2013. International Journal of Oral Biology. 10.11620/IJOB.2013.38.2.081

● (845) Kasuyama, Kenta; Tomofuji, Takaaki; Ekuni, Daisuke; Tamaki, Naofumi; Azuma, Tatsuji; Irie, Koichiro; Endo, Yasumasa; Morita, Manabu. Hydrogen-rich water attenuates experimental periodontitis in a rat model. 2011. Journal of Clinical Periodontology. 10.1111/j.1600-051X.2011.01801.x

● (847) Kim, Young-Mi; Choi, Yu-Sung; Cho, In-Ho. Cleansing effect of the alkaline ionized water on microorganisms of the denture surface. 2011. Journal of Korean Academy of Prosthodontics. 10.4047/jkap.2011.49.2.138

● (864) Okajima, Masahiro; Kanzaki, M.; Ishibashi, Y.; Wada, Y.; hino, F.; Kitahara, Y.; Shimokawa, K.; Ishii, Fumiyoshi. In vitro bactericidal activity against periodontopathic bacteria by electrolyzed ion-reduced water. 2011. Drug Discoveries and Therapeutics. 10.5582/ddt.2011.v5.6.306

● (945) Saitoh, Yasukazu; Yoshimura, Y.; Nakano, K.; Miwa, Nobuhiko. Platinum nanocolloid-supplemented hydrogen-dissolved water inhibits growth of human tongue carcinoma cells preferentially over normal cells. 2009. Experimental Oncology. 19783965

● (959) Saitoh, Yasukazu; Okayasu, Hajime; Xiao, Li; Harata, Yoshikazu; Miwa, Nobuhiko. Neutral pH Hydrogen-Enriched Electrolyzed Water Achieves Tumor-Preferential Clonal Growth Inhibition Over Normal Cells and Tumor Invasion Inhibition Concurrently With Intracellular Oxidant Repression. 2008. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics. 10.3727/096504008786991620

● (974) Lee, Sung-Hoon; Choi, Bong-Kyu. Antibacterial effect of electrolyzed water on oral bacteria. 2006. Journal of Microbiology. 16953177

● (1008) Horiba, Naoki; Hiratsuka, Kouiti; Onoe, Takaya; Yoshida, Tsutomu; Suzuki, Kazuyoshi; Matsumoto, Toru; Nakamura, Hiroshi. Bactericidal effect of electrolyzed neutral water on bacteria isolated from infected root canals. 1999. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 10.1016/S1079-2104(99)70300-8

● (1033) Nayak, Aarati; Bhatt, Akanksha; Bhat, Kishore; Nayak, Ranganath; Hooli, Amruta; Naik, Sachita. Assessment of antibacterial effect of hydrogen water on plaque from patients with chronic periodontitis. 2021. Journal of the Indian Society of Periodontology. 10.4103/jisp.jisp_317_20

● (1102) Saitoh, Yasukazu; Yonekura, Nene; Matsuoka, Daigo; Matsumoto, Akira. Molecular hydrogen suppresses Porphyromonas gingivalis lipopolysaccharide-induced increases in interleukin-1 alpha and interleukin-6 secretion in human gingival cells. 2021. Molecular and Cellular Biochemistry. 10.1007/s11010-021-04262-7