Studies Referencing Hydrogen and Aging/Lifespan

Database:

Primary Topic: Any

Secondary Topic: Aging

Tertiary Topic: Life Expectancy/Telomere Length (or Any)

● Hydrogen applied to aging skin has been shown to

• increase thickness (90: Kwon, Han, and Park 2020)
• and moisture (90: Kwon, Han, and Park 2020),
• decrease sebum content (90: Kwon, Han, and Park 2020)
• and cellular markers of skin aging (i.e. disulfide bridges) (441: Wu et al 2017),
• and lower the ORP (Oxidation-Reduction Potential) (989: Okouchi et al 2003).

● Hydrogen improves markers of cellular senescence and molecular aging, including

• increasing the activity of telomerase and the length of chromosomal telomeres (102: Logozzi et al 2020; 514: Settineri et al 2016; 1099: Zanini et al 2021),
• age-related changes in DNA methylation (1099: Zanini et al 2021),
• and decreasing the expression of senescence-related proteins (i.e. p53/p21) (360: Zhang et al 2018; 825: Chen et al 2011; 244: Wu et al 2019; 310: Li et al 2018; 272: Chi et al 2018; 326: Paulis et al 2018; 1152: Li et al 2019)
• and markers (i.e. β-galactosidase) (360: Zhang et al 2018; 389: Han, Park, and Park 2017; 1110: Liu et al 2021)

● Hydrogen has been shown to increase the lifespan of

• fruit flies (205: Klichko et al 2019),
• nematode worms (685: Zhang et al 2014; 726: Park and Park 2013; 880: Yan et al 2011; 924: Yan et al 2010; 925: Yan et al 2010),
• and mice (503: Magro et al 2016; 1013: Nelson et al 1998).

● Hydrogen protects

• the kidneys (Xu et al 2019; 756: Zhu et al 2013),
• periodontal tissues (668: Tomofuji et al 2014),
• eyes (1106: Liang et al 2021),
• brain (876: Ueda, Kojima, and Oikawa 2011; 895: Gu et al 2010; 86: Korovljev et al 2020; 638: Kashiwagi et al 2014; 1099: Zanini et al 2021; 1116: Shinada et al 2021),
• and heart (302: Korovljev et al 2018; 295: Iketani et al 2018; 756: Zhu et al 2013; 482: Hara et al 2016; 574: Pan et al 2015) against aging.

● Hydrogen is cardioprotective in the middle-aged and elderly, lowering American Heart Association (AHA) risk scores, increasing cardiorespiratory fitness, controlling blood sugar, lowering cholesterol and triglycerides, and lowering body fat percentage (302: Korovljev et al 2018; 303: Korovljev et al 2018)

● Hydrogen supplementation has been shown to increase physical fitness in the elderly (302: Korovljev et al 2018).

Literature Cited (numbered as per database):

● (86) Korovljev, Darinka; Trivic, Tatjana; Stajer, Valdemar; Drid, Patrik; Sato, Bunpei; Ostojic, Sergej M. Short-Term H2 Inhalation Improves Cognitive Function in Older Women: A Pilot Study. 2020. International Journal of Gerontology. 10.6890/IJGE.202005_14(2).0013

● (90) Kwon, Hye-Jin; Han, Sang-Beom; Park, Kyung-Won. Antioxidant Activity of Hydrogen Water Mask Pack Composed of Gel-Type Emulsion and Hydrogen Generation Powder. 2020. International Journal of Molecular Sciences. 10.3390/ijms21249731

● (102) Logozzi, Mariantonia; Mizzoni, Davide; Di Raimo, Rosella; Andreotti, Mauro; Macchia, Daniele; Spada, Massimo; Fais, Stefano. In vivo antiaging effects of alkaline water supplementation. 2020. Journal of Enzyme Inhibition and Medical Chemistry. 10.1080/14756366.2020.1733547

● (205) Klichko, Vladimir I.; Safonov, Vladimir L.; Safonov, Marina Yu.; Radyuk, Svetlana N. Supplementation with hydrogen-producing composition confers beneficial effects on physiology and life span in Drosophila. 2019. Heliyon 10.1016/j.heliyon.2019.e01679

● (244) Wu, Guojiao; Chen, Zhiheng; Wang, Peipei; Zhao, Mingyi; Fujino, Masayuki; Zhang, Chen; Zhou, Wenjuan; Hirano, Shin-Ichi; Li, Xiao-Kang; Zhao, Lingling. Hydrogen inhalation protects hypoxic–ischemic brain damage by attenuating inflammation and apoptosis in neonatal rats. 2019. Experimental Biology and Medicine. 10.1177/1535370219855399

● (245) Xu, Xianlin; He, Xiaozhou; Liu, Jipu; Qin, Jiandi; Ye, Jianan; Fan, Min. Protective effects of hydrogen-rich saline against renal ischemia-reperfusion injury by increased expression of heme oxygenase-1 in aged rats. 2019. International Journal of Clinical and Experimental Pathology. 31933966

● (272) Chi, Jing; Li, Zizhou; Hong, Xiaojian; Zhao, Tong; Bie, Yueyue; Zhang, Wen; Yang, Jiaxing; Feng, Ziming; Yu, Zhouqi; Xu, Qiannan; Zhao, Luqi; Liu, Weifan; Gao, Yunan; Yang, Hongxiao; Yang, Jiemei, Liu, Jiaren; Yang, Wei. Inhalation of Hydrogen Attenuates Progression of Chronic Heart Failure via Suppression of Oxidative Stress and P53 Related to Apoptosis Pathway in Rats. 2018. Frontiers in Physiology. 10.3389/fphys.2018.01026

● (295) Iketani, Masumi; Sekimoto, Kanako; Igarashi, Tsutomu; Takahashi, Mayumi; Komatsu, Masaki; Sakane, Iwao; Takahashi, Hiroshi; Kawaguchi, Hideo; Ohtani-Kaneko, Ritsuko; Ohsawa, Ikuroh. Administration of hydrogen-rich water prevents vascular aging of the aorta in LDL receptor-deficient mice. 2018. Nature Scientific Reports. 10.1038/s41598-018-35239-0

● (302) Korovljev, Darinka; Stajer, Valdemar; Javorac, Dejan; Ostojic, Sergej M. Hydrogen inhalation positively affects cardiometabolic risk factors in men and women aged 65 years or older: a preliminary report. 2018. European Geriatric Medicine. 10.1007/s41999-018-0087-6

● (303) Korovljev, Darinka; Trivic, T.; Drid, P.; Ostojic, Sergej M. Molecular hydrogen affects body composition, metabolic profiles, and mitochondrial function in middle-aged overweight women. 2018. Irish Journal of Medical Science. 10.1007/s11845-017-1638-4

● (310) Li, Shaowei; Fujino, Masayuki; Ichimaru, Naotsugu; Kurakawa, Ryosuke; Hirano, Shinichi; Mou, Lisha; Takahara, Shiro; Takahara, Terumi; Li, Xiao-Kang. Molecular hydrogen protects against ischemia-reperfusion injury in a mouse fatty liver model via regulating HO-1 and Sirt1 expression. 2018. Nature Scientific Reports. 10.1038/s41598-018-32411-4

● (326) Paulis, Melad G.; Hassan, Osama A.; Abbass, Mohmed F.; Mohammad, Mostafa Abo-Al Hamad. Structural and lipid peroxidation effects of lead on rat hippocampus and its attenuation by hydrogen rich water. 2018. Journal of Chemical Neuroanatomy. 10.1016/j.jchemneu.2018.04.004

● (360) Zhang, Wenbo; Huang, Chao; Sun, Aijun; Qiao, Liang; Zhang, Xi; Huang, Junlong; Sun, Xuejun; Yang, Xiangqun; Sun, Shanquan. Hydrogen alleviates cellular senescence via regulation of ROS/p53/p21 pathway in bone marrow-derived mesenchymal stem cells in vivo. 2018. Biomedicine and Pharmacotherapy. 10.1016/j.biopha.2018.07.020

● (389) Han, A. Lum; Park, Seong-Hoon; Park, Mi Sung. Hydrogen Treatment Protects against Cell Death and Senescence Induced by Oxidative Damage. 2017. Journal of Microbiology and Biotechnology. 10.4014/jmb.1608.08011

● (441) Wu, Ching-Ying; Hsu, Wen-Li; Tsai, Ming-Hsien; Liang, Jui-Lin; Lu, Jian-He; Yen, Chia-Jung; Yu, Hsin-Su; Noda, Mami; Lu, Chi-Yu; Chen, Chu-Huang; Yan, Shain-Jang; Yoshioka, Tohru. Hydrogen gas protects IP3Rs by reducing disulfide bridges in human keratinocytes under oxidative stress. 2017. Nature Scientific Reports. 10.1038/s41598-017-03513-2

● (482) Hara, Fumihiko; Tatebe, Junko; Watanabe, Ippei; Yamazaki, Junichi; Ikeda, Takanori; Morita, Toshisuke. Molecular Hydrogen Alleviates Cellular Senescence in Endothelial Cells. 2016. Circulation Journal. 10.1253/circj.CJ-16-0227

● (503) Magro, Massimiliano; Corain, Livio; Ferro, Silvia; Baratella, Davide; Bonaiuto, Emanula; Terzo, Milo; Corraducci, Vittorino; Salmaso, Luigi; Vianello, Fabio. Alkaline Water and Longevity: A Murine Study. 2016. Evidence-Based Complementary and Alternative Medicine. 10.1155/2016/3084126

● (514) Settineri, Robert; Ji, Jin; Luo, Chunlan; Ellithorpe, Rita R.; de Mattos, Gonzalo Ferreira; Rosenblatt, Steven; La Valle, James; Jimenez, Antonio; Ohta, Shigeo; Nicolson, Garth L. Effects of Hydrogenized Water on Intracellular Biomarkers for Antioxidants, Glucose Uptake, Insulin Signaling and SIRT 1 and Telomerase Activity. 2016. American Journal of Food and Nutrition. 10.12691/ajfn-4-6-4

● (574) Pan, Z.; Zhao, Y.; Yu, H.; Liu, D.; Xu, H. Effect of hydrogen-rich saline on cardiomyocyte autophagy during myocardial ischemia-reperfusion in aged rats. 2015. National Medical Journal of China. 26710815

● (638) Kashiwagi, Taichi; Yan, Hanxu; Hamasaki, Takeki; Kinjo, Tomoya; Nakamichi, Noboru; Teruya, Kiichiro; Kabayama, Shigeru; Shirahata, Sanetaka. Electrochemically reduced water protects neural cells from oxidative damage. 2014. Oxidative Medicine and Cellular Longevity. 10.1155/2014/869121

● (668) Tomofuji, Takaaki; Kawabata, Yuya; Kasuyama, Kenta; Endo, Yasymasa; Yoneda, Toshiki; Yamane, Mayu; Azuma, Tatsuji; Ekuni, Daisuke; Morita, Manabu. Effects of hydrogen-rich water on aging periodontal tissues in rats. 2014. Nature Scientific Reports. 10.1038/srep05534

● (685) Zhang, Miao; Li, Zhihui; Gao, Dawen; Gong, Wenjing; Gao, Wan; Zhang, Cheng-Gang. Hydrogen extends Caenorhabditis elegans longevity by reducing reactive oxygen species. 2014. PLoS One. 10.1371/journal.pone.0231972

● (726) Park, Seul-Ki; Park, Sang-Kyu. Electrolyzed-reduced water increases resistance to oxidative stress, fertility, and lifespan via insulin/IGF-1-like signal in C. elegans. 2013. Biological Research. 10.4067/S0716-97602013000200005

● (756) Zhu, Wan-Jun; Nakayama, Masaaki; Mori, Takefumi; Hao, Kiyotaka; Terawaki, Hiroyuki; Katoh, Junichiro; Kabayama, Shigeru; Ito, Sadayoshi. Amelioration of cardio-renal injury with aging in dahl salt-sensitive rats by H2-enriched electrolyzed water. 2013. Medical Gas Research. 10.1186/2045-9912-3-26

● (825) Chen, Da-Lung; Chen, Tzen-Wen; Chien, Chiang-Ting; Li, Ping-Chia. Intravenous low redox potential saline attenuates FeCl3-induced vascular dysfunction via downregulation of endothelial H2O2, CX3CL1, intercellular adhesion molecule-1, and p53 expression. 2011. Translational Research. 10.1016/j.trsl.2010.12.012

● (876) Ueda, Yuto; Kojima, Toshio; Oikawa, Taneaki. Hippocampal gene network analysis suggests that coral calcium hydride may reduce accelerated senescence in mice. 2011. Nutrition Research. 10.1016/j.nutres.2011.09.011

● (880) Yan, Hanxu; Kinjo, Tomoya; Tian, Huaize; Hamasaki, Takeki; Teruya, Kiichiro; Kabayama, Shigeru; Shirahata, Sanetaka. Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced water--participation of Pt nanoparticles. 2011. Bioscience, Biotechnology, and Biochemistry. 10.1271/bbb.110072

● (895) Gu, Yeunhwa; Huang, Chein-Sheng; Inoue, Tota; Yamashita, Takenori; Ishida, Torao; Kang, Ki-Mun; Nakao, Atsunori. Drinking hydrogen water ameliorated cognitive impairment in senescence-accelerated mice. 2010. Journal of Clinical Biochemistry and Nutrition. 10.3164/jcbn.10-19

● (924) Yan, Hanxu; Tian, Huaize; Hamasaki, Takeki; Abe, Masumi; Nakamichi, Noboru; Teruya, Hiichiro; Katakura, Yoshinori; Morisawa, Shinkatsu; Shirahata, Sanetaka. Electrolyzed Reduced Water Prolongs Caenorhabditis elegans’ Lifespan. 2010. Animal Cell Technology: Basic and Applied Aspects. 10.1007/978-90-481-3892-0_48

● (925) Yan, Hanxu; Tian, Huaize; Kinjo, Tomoya; Hamasaki, Takeki; Tomimatsu, Kosuke; Nakamichi, Noboru; Teruya, Kiichiro; Kabayama, Shigeru; Shirahata, Sanetaka. Extension of the lifespan of Caenorhabditis elegans by the use of electrolyzed reduced water. 2010. Bioscience, Biotechnology, and Biochemistry. 10.1271/bbb.100250

● (989) Okouchi, Shoichi; Takezaki, Daisuke; Ohnami, Hideyuki; Agishi, Yuhkoh; Kanroji, Yasuo; Ikeda, Shigeo. Electrolyzed-Reduced Water as Artificial Hot Spring Water. 2003. Journal of the Balneological Society of Japan. https://www.researchgate.net/publication/242555470_Electrolyzed-Reduced_Water_as_Artificial_Hot_Spring_Water

● (1013) Nelson, D.; Reddy Avula, C.P.; Jolly, C.; DeVierville, J.; Fernandes, Gabriel. Effect of electrolyzed water intake on lifespan of autoimmune disease prone mice. 1998. FASEB Journal. https://scholars.uthscsa.edu/en/publications/effect-of-electrolyzed-water-intake-on-lifespan-of-autoimmune-dis

● (1099) Zanini, Dragana; Todorovic, Nikola; Korovljev, Darinka; Stajer, Valdemar; Ostojic, Jelena; Purac, Jelena; Kojic, Danijela; Vukasinovic, Elvira; Djordjievski, Srdjana; Sopic, Miron; Guzonjic, Azra; Ninic, Ana; Erceg, Sanja; Ostojic, Sergej M. The effects of 6-month hydrogen-rich water intake on molecular and phenotypic biomarkers of aging in older adults aged 70 years and over: A randomized controlled pilot trial. 2021. Experimental Gerontology. 10.1016/j.exger.2021.111574

● (1106) Liang, I-Chia; Ko, Wen-Chin; Hsu, Yu-Jou; Lin, Yi-Ru; Chang, Yun-Hsiang; Zong, Hv-Hui; Lai, Pei-Chen; Chang, Der-Chen; Hung, Chi-Feng. The Anti-Inflammatory Effect of Hydrogen Gas Inhalation and Its Influence on Laser-Induced Choroidal Neovascularization in a Mouse Model of Neovascular Age-Related Macular Degeneration. 2021. International Journal of Molecular Sciences. 10.3390/ijms222112049

● (1110) Liu, Boyan; Xie, Yunbo; Chen, Jun; Xue, Junli; Zhang, Xiaoyi; Zhao, Min; Jia, Xiubin; Wang, Yiwei; Qin, Shucun. Protective Effect of Molecular Hydrogen Following Different Routes of Administration on D-Galactose-Induced Aging Mice. 2021. Journal of Inflammation Research. 10.2147/JIR.S332286

● (1116) Shinada, Takamitsu; Takano, Yuji; Kokubun, Keisuke; Iki, Hikari; Taki, Yasuyuki. Effects of Natural Reduced Water on Cognitive Function, Body Composition, and Psychological Function in Older Adults: Study Protocol for a Randomized Controlled Trial. 2021. Methods and Protocols. 10.3390/mps4040073

● (1152) Li, Ruichan; Liu, Yanli; Xie, Jing; Huang, Xudong; Zhang, Li; Liu, Hua; Li, Lihua. Sirt3 mediates the protective effect of hydrogen in inhibiting ROS-induced retinal senescence. 2019. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2019.02.005